Microscopy
Whether working with fluorescence, Raman, or nonlinear techniques, the ability to fine-tune the laser output to match the specific absorption or emission characteristics of the material being studied significantly enhances image resolution, contrast, and sensitivity.
In microscopy, our OPO and laser systems facilitate life sciences and material studies breakthroughs. Single-molecule microscopy enables the observation of individual biomolecules, revealing key information about molecular interactions. Multi-photon and nonlinear microscopy allow deep tissue imaging with minimal photodamage, ideal for super-resolution biological research. Techniques like time-resolved fluorescence microscopy provide time-sensitive insights, while linear fluorescence and Raman microscopy focus on material properties and molecular structures with unmatched detail.
Multi-Photon and Nonlinear Microscopy
Multi-photon microscopy uses two-photon or three-photon excitation to image biological tissues at greater depths with reduced photodamage. Ultrashort pulse lasers facilitate efficient fluorophore excitation while minimising the energy imparted to samples. Nonlinear microscopy — encompassing Second Harmonic Generation, Third Harmonic Generation and multi-photon processes — provides deeper tissue imaging and higher resolution without phototoxicity effects.
Raman and Fluorescence Microscopy
Raman microscopy is a label-free imaging technique providing molecular information based on vibrational modes, with CARS and SRS as advanced variations. Tunable lasers enable precise adjustment of the laser wavelength to match the vibrational frequencies of specific molecular bonds. In time-resolved fluorescence microscopy (FLIM) and linear fluorescence microscopy, tunable lasers allow researchers to select excitation wavelengths that match the absorption peaks of the fluorophores being studied, increasing sensitivity and image quality.
Optimising your microscopy set-up?
Our team can advise on the right broadly tunable laser system for your imaging technique, from multiphoton to Raman microscopy.
Relevant Resources
Background-Free 3D Nanometric Localization and Sub-nm Asymme…
Despite many advances, rapid, precise 3D tracking of nanoscale particles in a complex environment remains chall……
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Visible-wavelength two-photon excitation microscopy with mul…
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A large-field polarisation-resolved laser scanning microscop…
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Two-Photon Lithography of 3D Nanocomposite Piezoelectric Sca…
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Ultrafast Electron Cooling and Decay in Monolayer WS2 Reveal…
by time- and energy-resolved photoemission electron microscopy (PEEM). Electron cooling in the Q valley of the ……
Preserving the Emission Lifetime and Efficiency of a Monolay…
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Second harmonic generation in monolithic gallium phosphide m…
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Near-field terahertz nonlinear optics with blue light
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Spatially engineered nonlinearity in resonant metasurfaces
Spatial engineering of the nonlinear susceptibility χ ( 2 ) in resonant metasurfaces offers a new degree of fr……
Correlative light-electron microscopy using small gold nanop…
Correlative light-electron microscopy (CLEM) requires the availability of robust probes which are visible both ……
Simultaneous microscopic imaging of thickness and refractive…
The detection of spatial or temporal variations in very thin samples has\nimportant applications in the biologi……
Selective activation of cholinergic neurotransmission from t…
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Homo-FRET Based Biosensors and Their Application to Multiple…
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Explore our products
CW Laser Systems
Continuous-Wave broadly tunable laser systems with integrated pump laser and OPO. Hands-free wavelength tuning in the Near-IR and Mid-IR across 1450 – 4000 nm with superior power and stability.
Ultra Short Pulse Lasers
Femtosecond and picosecond broadly tunable laser systems with integrated pump laser and OPO. Hands-free wavelength tuning in the Near-IR and Mid-IR across 1400 – 7000 nm with superior power and stability.
Optical Parametric Oscillators – OPOs
Femtosecond OPOs pumped by mode-locked Ti:Sapphire lasers. Hands-free rapid wavelength tuning in the Visible, Near-IR and Mid-IR gap-free across 340 – 4000 nm.
Second Harmonic Generators
Harmonic generators to double the frequency of external mode-locked femtosecond and picosecond Ti:Sapphire lasers and femtosecond IR OPOs. Spectral coverage across 340 – 750 nm. Best-in-class conversion efficiency. Hands-free operation and plug-and-play installation.
What our customers say about us
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Professor Romain Quidant
ICFO, Spain
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Dr Pier Sazio
University of Southampton, United Kingdom, Senior Research Fellow, Optoelectronics Research Centre
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Radiantis were very flexible with our requirements and perfectly matched to our technical needs and budget. Radiantis OPO has enabled us to carry out advanced optical characterisation of our systems across the UV, Visible and IR, and has proven to be a very reliable and easy-to-use system, delivering high and stable power and fast automated tuning across the complete spectral range. Radiantis team were very friendly and provided a professional and comprehensive user training to key researchers in my lab, which enabled us to operate the OPO successfully and reliably over many years.
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The wide tuning range of our Radiantis OPO system has allowed us to access wavelength regimes that have enabled a number of nonlinear optics experiments that would otherwise not have been possible. Radiantis have been very responsive and any problems have always been dealt with swiftly, with engineers being sent over to our lab for extended visits whenever required.
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